Wanderers' Under 18 side suffered a 3-1 reverse at the hands of Leicester City on Saturday morning.
The Foxes had experienced a similar start to the Whites during the opening months of the season, but the hosts were to start the brighter of the two sides with a goal from Ben Chilwell inside four minutes of the clash.
With an early lead under their belts, Leicester were to hit Wanderers with a sucker-punch shortly after as Simonas Stankevicius doubled their advantage.
Not to be deterred however, the Whites enjoyed a number of opportunities to pull themselves back into the clash as Tom Walker hit the post with a free-kick and Jamie Thomas being adjudged to have been offside as he fired home the rebound.
Bolton's determination was clear to see, and their hard work was rewarded in the 65th minute as Carl Kennedy connected with Walker's corner to half the deficit.
A late rally saw Wanderers go close to snatching what would have been a deserved equaliser, but the Foxes hit them on the counter-attack to put the result beyond doubt.
Speaking following the final whistle, coach David Lee admitted his disappointment that his side had been forced to leave Belvoir Drive empty handed.
"We weren't good enough in the first half, but we were the better side in the second and deserved something out of the game," he explained. "There were a lot of positives that we can take away though and one or two great individual performances.
"We'll pick ourselves up and will be ready to go again against Newcastle on Saturday."
Bolton: Campbell, Greenaugh, Gibson, Ball, Kennedy, Cvetko, Thomas, Bailey, Abbotts (Nelson, 80), Hinchcliffe (Newby, 60), Walker