Stuart Attwell will oversee Wanderers' Sky Bet Championship clash with Sheffield United on Tuesday night.
An experienced Premier League official, the Warwickshire-based referee will be running the rule over his 14th Whites fixture.
Mr Attwell last took charge a Bolton game last season when the Trotters were knocked out of the Emirates FA Cup by Crystal Palace back in January.
He has refereed two games so far this campaign, dishing out three yellow cards and no red cards, while he issued 90 yellow cards and eight red cards from 27 games last season.
Steve Child and Marc Perry will run the line while Andrew Madley is the fourth official.