Wanderers will face Fleetwood Town in the Papa John’s Trophy at the University of Bolton Stadium on Tuesday, 30 November (KO 7pm).
Ian Evatt’s men were drawn against Fleetwood in the northern section of the competition after topping their qualifying group with a 100 per cent winning record.
The last-32 fixture will be the first of two meetings between the two sides in the space of eight days with Wanderers going to Fleetwood for a League One game on Tuesday, 7 December.
Fleetwood reached the knock-out stages of the northern section by finishing second in Group G behind Accrington Stanley after beating Barrow and Leicester City’s Under-21s.
Ticket information for the tie will be announced in due course.