Josh Sheehan returns to the side and captains Wanderers as the Whites look to close the gap on the play-off places.
Sheehan starts again in midfield as one of four changes made by head coach Steven Schumacher to the side that made it successive league wins at Shrewsbury Town a week ago.
George Thomason is back from a ban to be named among the substitutes, with Eoin Toal and Gethin Jones absent through injury.
Widemen Jordi Osei-Tutu and Szabi Schön are re-called with Josh Dacres-Cogley also returning to the starting line-up.
Leyton Orient, who are currently three points ahead Wanderers in the final play-off spot, include former Whites’ wide player Randell Williams but are without suspended skipper and ex-Wanderer Darren Pratley.
Wanderers: Southwood, Murphy, Johnston, Sheehan, Dacres-Cogley, Osei-Tutu, Forrester, Morley, Schön, Matete, McAtee. Substitutes: Baxter, Forino, Thomason, Collins, Lolos, Adeboyejo, Randall.
Leyton Orient: Keeley, Cooper, Brown, Williams, Currie, Ball, Donley, Galbraith, Kelman, Edmonds-Green, Abdulai. Substitutes: Phillips, Sweeney, Agyei, Perkins, Jaiyesimi, Obiero, Markanday.
Referee: Sam Purkiss